Extended Data Fig. 5: Properties of ITCdm and ITCvm neurons during fear extinction.
From: Intercalated amygdala clusters orchestrate a switch in fear state
a, Trial-averaged ΔF/F Ca2+ responses of ITCdm fear and ITCvm extinction neurons aligned to freezing onset and offset. b, Schematics illustrating the analysis shown c. Correlation coefficients between trial-by-trial (in total, 50 CS trials) freezing levels and CS-response amplitudes of all the recorded neurons across two days of extinction. Distributions of those correlation coefficients (one value from each neuron) were first normalized in each animal, then mean ± s.e.m. values were acquired from all ITCdm and ITCvm recordings and visualized. c, The distribution of correlation coefficients for ITCdm neurons was skewed towards 1, indicating that a larger fraction showed a CS response pattern positively correlated with freezing behaviour. By contrast, ITCvm neurons exhibited the opposite tendency; a response pattern anti-correlated with freezing behaviour. *P = 1.64 × 10−40, Kolmogorov–Smirnov test. d, Trial-averaged ΔF/F Ca2+ responses of ITCvm neurons aligned to tone on habituation (day 1). e, Left, averaged Ca2+ responses to tone CS onset in ITCdm. Vertical dotted lines indicate onsets and 2-s time-window of CSs for AUC analysis. Middle, single-trial average of all the ITCdm neurons. Right, AUC quantification of single-trial responses shown in the middle panel. P = 0.027, one-way ANOVA. f, Trial-averaged ΔF/F Ca2+ responses of ITCdm fear and ITCvm extinction neurons aligned to US omission on day 3. Early, CS1–5; late, CS21–25. Dotted red boxes indicate the expected timing of US delivery. g, Trial-averaged ΔF/F Ca2+ responses of all recorded ITCvm neurons aligned to US omission on day 5. The dotted red box indicates the expected timing of US delivery. h, Relationship between CS responses during last five trials of extinction training on day 4 and US omission responses on day 3 in ITCvm extinction neurons shows a weak positive correlation.
