Fig. 2: RBD–scNP elicits a higher titre of neutralizing antibodies against more transmissible or neutralization-resistant SARS-CoV-2 variants than does S-2P mRNA-LNP.
From: Neutralizing antibody vaccine for pandemic and pre-emergent coronaviruses
a, b, The location of K417, E484 and N501 (spheres), which are mutated in the B.1.351 variant, are shown in the cryo-electron microscopy structures of the RBD neutralizing antibodies DH1041 (red) (a) and DH1047 (magenta) (b) bound to the RBD (grey) of spike trimers (Protein Data Bank codes (PDB) 7LAA and 7LD1)15. c, ACE2 receptor, DH1041 and DH1047 enzyme-linked immunosorbent assay (ELISA) binding titre as log(AUC), for wild-type (WT) and mutant SARS-CoV-2 spike RBD monomers. d, Serum neutralization ID50 (left) and ID80 (right) titres for SARS-CoV-2 D614G and SARS-CoV-2 B.1.1.7 pseudoviruses from immunized macaques, in ACE2-expressing 293 cells. Symbols represent individual macaques; horizontal bars are group means. **P = 0.0079, two-tailed exact Wilcoxon test. n = 5 macaques. e, Fold decrease in neutralization potency in neutralization of SARS-CoV-2 B.1.1.7 pseudovirus relative to SARS-CoV-2 D614G pseudovirus. Fold change is shown for RBD–scNP-immunized and S-2P mRNA-LNP-immunized macaques on the basis of the ID50 and ID80 titres. Horizontal bars are the group mean. f, Vaccinated macaque serum neutralization ID50 (left) and ID80 titres (right) against SARS-CoV-2 WA-1 and B.1.351 pseudoviruses, in ACE2-expressing 293 cells. Symbols and horizontal bars are shown the same as in d. *P = 0.0159; **P = 0.0079, two-tailed exact Wilcoxon test. n = 5 macaques. g, Fold decrease in neutralization potency in neutralization of SARS-CoV-2 B.1.351 pseudovirus relative to SARS-CoV-2 WA-1 pseudovirus. Fold change is shown as in e. h, Vaccine-induced ID50 (left) and ID80 (right) neutralization titres of infection of ACE2- and TMPRSS2-expressing 293 cells by SARS-CoV-2 WA-1 or P.1 pseudovirus. Symbols and horizontal bars are as in d. *P = 0.0159; **P = 0.0079, two-tailed exact Wilcoxon test. n = 5 macaques. i, Fold decrease in neutralization potency (as shown in e) for SARS-CoV-2 P.1 pseudovirus relative to SARS-CoV-2 WA-1 pseudovirus.
