Extended Data Fig. 4: Cross-nAbs are elicited by RBD–scNP and mRNA-LNP immunization.
From: Neutralizing antibody vaccine for pandemic and pre-emergent coronaviruses
a, Each row shows neutralization titre for an individual macaque immunized with one of the three immunogens. A reciprocal serum dilution titre of 87,480 is the upper limit of detection and 20 is the lower limit of detection for this assay. Titres are derived from a nonlinear regression curve fit to the average of duplicate measurements. b, c, Serum neutralization titres elicited by two S-2P mRNA-LNP immunizations were boosted by a subsequent RBD–scNP immunization. Serum neutralization of SARS-CoV-2 D614G (b) and SARS-CoV-2 B.1.1.7 (c) pseudovirus infection of ACE2-expressing 293 cells. Neutralization titres are ID50 as reciprocal serum dilution for serum collected two weeks after the second (week 6) and third immunization (week 10). Each symbol connected by a line represents the titre for an individual macaque before and after RBD–scNP immunization. Normal human serum spiked with DH1043 was used as a positive control.
