Extended Data Fig. 5: Cross-reactive plasma antibody responses elicited by RBD–scNP immunization in macaques.
From: Neutralizing antibody vaccine for pandemic and pre-emergent coronaviruses
a, Plasma IgG from macaques immunized twice with RBD–scNP binds to spike from human, bat and pangolin SARS-related coronavirus spike in ELISA, but not endemic human coronaviruses or MERS-CoV. b, Determination of DH1047 Fab binding kinetics to RBD monomer by surface plasmon resonance. Each curve shows a different concentration of DH1047 Fab. Binding kinetics are shown to the right from a 1:1 model fit. c, Time course of vaccinated macaque plasma IgG binding to human, bat and pangolin coronavirus spike protein by ELISA. Each curve indicates the binding titre for an individual macaque. Arrows indicate immunization time points. d, Unimmunized macaque plasma antibody blocking of SARS-CoV-2 S-2P (left) and SHC014 (middle) binding to ACE2, RBD neutralizing antibody DH1041 and RBD cross-nAb DH1047. Right, blocking activity in the serum of humans immunized with Pfizer BNT162b2 vaccine (n = 4). Each symbol represents an individual human or macaque. Bars indicate group mean ± s.e.m.
