Extended Data Fig. 3: Replication of strong protective effects in a larger group of mice vaccinated with candidate V23 (IFX).
From: An invariant Trypanosoma vivax vaccine antigen induces protective immunity
a, Quantification of replicate vaccinations and T. vivax infections with four antigens showing protective effects in the initial screen. V23 and V31 vaccinations replicated, but not V2 and V8. Parasitaemia was quantified on day six using bioluminescence; data points represent individual mice and bars indicate mean ± s.d. ns, not significant, ****P ≤ 0.0001 two-tailed t-test; grey shading indicates background bioluminescence thresholds. b, Fifteen mice were immunized with purified soluble IFX–V23 recombinant protein adjuvanted in alum and challenged with transgenic luciferase-expressing T. vivax. Parasitaemia was quantified on the indicated days after parasite challenge using bioluminescence; controls are a cohort of 15 mice treated with adjuvant only. Ten out of the 15 mice were protected until at least day 170. Data points represent individual mice and grey shading indicates bioluminescence thresholds of uninfected mice. Where mice had to be euthanized for health reasons thought to be unrelated to the infection, this is indicated by a cross.
