Extended Data Fig. 1: Red2Onco system: an oncogene-associated multicolour reporter.
From: Tracing oncogene-driven remodelling of the intestinal stem cell niche
a, Representative confocal images of mutant clones from sections (Red2-Notch1ICD) or whole mounts (Red2-KrasG12D and Red2-PIK3CAH1047R) of small intestine from Villin-CreERT2;Red2Onco mice 2 w after tamoxifen administration. Crypt borders are marked with a grey dashed outline. b, Average clone numbers collected from a single field of image (0.15 mm2) of whole-mount small intestine from Villin-CreERT2;R26R-Confetti or Red2Onco mice 2 d after tamoxifen administration. c–e, Representative confocal images (left) and quantification (right) of EdU+ proliferating crypt cells (c), LYZ+ Paneth cells (d) and MUC2+ goblet cells (e) from sections of small intestine from Villin-CreERT2;R26R-Confetti or Red2Onco mice 4 w after tamoxifen administration. f, Representative confocal images of 100-μm-thick sections or whole mounts of tissues from adult R26R-CreERT2; Red2Onco mice (skin and stomach corpus), Sftpc-CreERT2; Red2Onco mice (lung) or Krt5-CreERT2; Red2Onco mice (oesophagus) at 1, 2 and 4 w after tamoxifen administration. White dashed line, epithelial lining. β-catenin stained as a cell membrane marker. SPC marks alveolar type II cells in lung. g, Representative confocal images of sectioned mouse embryonic pancreas tissue from the R26R-CreERT2; Red2-KrasG12D mouse on embryonic day (E)18.5, 6 d after tamoxifen administration. Magnified panel to the right shows an example of acinar cell expansion in developing pancreas. CPA1 marks acinar cells. *P < 0.05, **P < 0.01, ***P < 0.0001 by one-way ANOVA with Games-Howell’s multiple comparisons test (b, c) or unpaired two-tailed t-test (d, e) from biological replicates. Data are mean ± s.d. (b) or mean ± s.e.m. (c–e). Exact P values are presented in Source Data. Scale bars: 50 μm (a, c–f) or 200 μm (g).
