Extended Data Fig. 8: Engraftment of transplanted Townes mouse HSPCs, clonality of editing outcomes, and oxygen binding affinity of blood.

a, Donor cell engraftment measured by flow cytometry assessing the percentage of CD45.2⁺ cells among PBMCs. b, Bone marrow from three mice transplanted with edited Townes mouse HSPCs was plated at low density in methylcellulose. After 12 days of culture, 30 to 35 individual colonies per mouse were picked into cell lysis buffer and the edited locus was amplified by PCR and sequenced by HTS. Colonies were categorized by whether they contained no editing, a monoallelic edit, or a biallelic edit. c, Blood was drawn from mice at week 14 after transplantation. Haemoglobin oxygenation was measured using a Hemox Analyzer (TCS Scientific) across a continuous declining gradient of oxygen pressure to assess whether HBB^S-to-HBB^G editing led to altered haemoglobin–oxygen binding. Data shown as mean ± s.d.