Extended Data Fig. 6: Extended data relating to C. difficile sufficiency experiments.
From: Caloric restriction disrupts the microbiota and colonization resistance
a, Experimental design relating to JBZPo1 transplantation experiment (Fig. 3b). b, Establishment of colonization with C. difficile JBZPo1 did not lead to dehydration as determined by hydration ratio (hydration ratio = [total body water − free water]/lean mass; P = 0.59, two-sided Mann–Whitney U test). Centre line, median; box, first and third quartiles; whiskers, 1.5× interquartile range (IQR) with outliers individually plotted. c, Body composition analysis revealed a significant difference between humanized vehicle control and C. difficile colonized mice at the end of the experiment (P < 0.001, LMM with Tukey’s two-sided all-pair comparison, n = 6 mice per group), which suggests that C. difficile caused increased adiposity. d, Quantification of JBZPo1 in recipient mice (P = 0.003–0.006, n = 6 mice per group per time point except n = 5 mice at 8 days in humanized control owing to missing sample; two-sided Mann–Whitney U test). Data shown as mean ± s.e.m. e, ELISA of caecal contents from recipient mice confirmed production of TcdA/TcdB only in JBZPo1 recipient mice at end of experiment. f, Blinded pathological analysis revealed minor neutrophil infiltration with reactive changes (two-sided Mann–Whitney U test, n = 6 mice per group).
