Extended Data Fig. 7: Diet-induced changes in microbiota influence C. difficile-associated weight loss and Toxin B expression.
From: Caloric restriction disrupts the microbiota and colonization resistance
a, Experimental design. b, Weight loss over time demonstrates a significant effect of diet of donor (n = 7–8 mice per group per day as in a; P = 5.9 × 10−5, estimate = −4.6% (−6.6 to −2.7 95% CI) for VLCD, P = 1.5 × 10−7, estimate = −6.6% (−8.5 to −4.6 95% CI) for CONVD). c, Mice colonized with stool samples from donors on CONVD showed an increase in TcdB expression over baseline 2 days after colonization (P = 0.003, n = 7–8 mice per group as in a, Kruskal–Wallis with two-sided Dunn’s test). d, C. difficile carriage did not differ significantly among groups (suggesting no modulation of virulence), with the exception of 8 days after colonization in CONVD-recipient mice (P = 0.017 CONVD versus baseline; n = 7–8 mice per group as in a; two-sided Mann–Whitney U test). Data shown as mean ± s.e.m. in c, d. e, Concentrations of key bile acids in P50 in response to diet (n = 1 participant per time point). LMM with participant as random effect and Tukey two-sided all-pair comparison unless otherwise noted.
