Extended Data Fig. 5: Characterization of C. difficile JBZPo1.
From: Caloric restriction disrupts the microbiota and colonization resistance
a, JBZPo1 was assembled with 255-fold coverage (Illumina MiSeq 250) into 120 contigs (inner grey track; N50 = 87,201 bp) with an average GC content of 28.6% (green–purple centre track showing GC content), and 3,777 coding sequences (outer red and blue tracks displaying positive and negative strands, respectively). b, The C. difficile pathogenicity locus (PaLoc) encodes both toxin A (tcdA) and B (tcdB). c, The binary toxin (cdt) locus (CdtLoc) does not encode intact binary toxin. d, Phylogenetic tree of 717 C. difficile genomes and associated virulence factor carriage places JBZPo1 flanked by Ribotype 014-20 strains and separate from the hypervirulent epidemic NAP1/B1/027 strains (for example, R20291).
