Fig. 1: Early kinetic and structural intermediate of tRNA2–mRNA translocation.
From: Structural basis of early translocation events on the ribosome
a, Schematic of the translocation reaction coordinate in bacteria depicting SSU body-rotation (blue) and head-swivel (purple). tRNAs are coloured on a gradient from the A (green) to P (yellow) to E (orange) sites. The states enclosed in dashed boxes were characterized in this study. Green (donor, uS13, LD550) and red (acceptor, uL1, LD650) circles denote fluorophore positions (see b). FA, fusidic acid. b, Population FRET histograms showing FRET evolution over time upon EF-G injection with buffer, SPC (3 mM) or fusidic acid (FA, 400 μM). n represents the number of observed molecules. c, Overview of the INT1 ribosome structure captured by SPC, coloured as in a.
