Extended Data Fig. 2: Fourier shell correlation and local resolution for cryo-EM structures along the translocation reaction coordinate.
From: Structural basis of early translocation events on the ribosome
a–f, Fourier shell correlation (FSC) resolution curves for PRE-C (a), PRE-H2* (b), PRE-H1 (c), POST (d), INT1 (e) and INT2 (f) structures obtained by masking the two half maps and calculating the cross-resolution between the masked volumes in Relion. Resolution was estimated using the 0.143 cut-off criterion (dotted line). g–l, Local resolution electron density maps for PRE-C (g), PRE-H2* (h), PRE-H1 (i), POST (j), INT1 (k) and INT2 (l). CP, central protuberance. LSU, grey background; SSU, blue background. Threshold σ = 5. See also Methods and Supplementary Table 1.
