Extended Data Fig. 10: COV2-2130/COV2-2196 antibody cocktail protects hamsters against historical and variant SARS-CoV-2 strains.
From: In vivo monoclonal antibody efficacy against SARS-CoV-2 variant strains
Six-week-old male Syrian golden hamsters received a single 800 μg (about 10 mg kg−1) (a–h) or 320 μg (about 4 mg kg−1) dose (i–p) of COV2-2130/COV2196 mAb cocktail or control mAb by intraperitoneal injection one day before intranasal inoculation with 5 × 105 FFU of WA1/2020 D614G or Wash-B.1.351 viruses. Nasal swabs and lung tissues were collected at three and four days after infection, respectively. a, i, Weight change following infection with SARS-CoV-2 (line indicates mean; n = 5 hamsters per group, one experiment). Infectious virus in the lung (b, j) or viral RNA levels in the lung (c, k) and nasal swabs (d, l) were determined (line indicates median; n = 5 hamsters per group, one experiment). Dotted line indicates the limit of detection of the assay. e–h, m–p, Cytokine and inflammatory gene expression in lung homogenates collected at 6 days after infection from indicated groups (line indicates mean; n = 5 hamsters per group). Values were calculated using the ΔΔCt method compared to a naive control group. Because data were obtained from a single experiment (even with multiple hamsters), statistical analysis was not performed.
