Extended Data Fig. 10: NDV-3A induces an intestinal anti-Als3 antibody response.
From: Adaptive immunity induces mutualism between commensal eukaryotes
a, model of monocolonization and DSS experiment in vaccinated mice. b, c, ELISA quantification of Als3-specific IgA (b) and IgG (c) from the faeces of GF mice one week after boost with alum of NDV-3A vaccine. d, e, Faecal (d) and intestinal (e) lumen CFU of C. albicans in monocolonized alum or NDV-3A vaccinated mice. Intestinal CFU quantified 12 days after colonization. f, Imaging flow cytometry images of IgA+ C. albicans from caecum of NDV-3A vaccinated mice. g, Percentage of hyphae quantified using an AF488 anti-Candida antibody to visualize morphology from indicated intestinal region 12 days after monocolonization. h, HWP1 and HYR1 transcripts quantified by qRT–PCR from colon C. albicans 12 days after monocolonization. (for b–h, n = 5 mice per group; one experiment). i, j, ELISA quantification of Als3-specific IgA (i) and IgG (j) in the faeces of conventionally colonized mice used for the DSS experiment. k, C. albicans CFU in colon contents after DSS treatment (for i–k, n = 10 mice per group, one experiment). l, Example H&E-stained histology images from the NDV-3A DSS experiment. P values calculated using two-way ANOVA with Sidak’s test (b, c, i, j). All data are mean ± s.d. Silhouettes in a were created using BioRender.
