Extended Data Fig. 1: Human faecal and serum anti-fungal antibodies.
From: Adaptive immunity induces mutualism between commensal eukaryotes
a, Human faecal antibody binding to cultured fungi and quantified by flow cytometry after staining with fluorescent secondary antibodies (n = 70). Staining intensity normalized to fungi stained with secondary antibodies but without human faecal wash. Box plots show minimum 25% quartile and maximum 75% quartile around the median and whiskers show range. b, IgA binding to cultured fungi from serial dilutions of human faecal wash (n = 30 healthy, n = 23 Crohn’s disease and n = 17 UC). Geometric mean with 95% CI c, d, Human serum antibody binding to cultured fungi. Serum diluted 1:75. (n = 12, n = 4 healthy and n = 8 Crohn’s disease). Mean ± s.d. e, Faecal ASCA IgA levels from undiluted faecal wash (n = 30 healthy, n = 18 Crohn’s disease and n = 14 UC). Median with 95% CI f, Serum ASCA IgA levels from human serum samples diluted 1:100 (n = 4 healthy and n = 8 Crohn’s disease). Median with 95% CI. P values calculated using two-way ANOVA with Tukey’s test (a), one-way ANOVA with Dunn’s test (c) or two-sided Mann–Whitney U-test (f, d).
