Extended Data Fig. 5: Filamentation and Ahr1 promote intestinal IgA responses.
From: Adaptive immunity induces mutualism between commensal eukaryotes
a, Morphology of indicated C. albicans strains incubated for 4 h in RPMI with 10% FBS, YPD or YPD + 5 μg ml−1 aTC). TetO-NRG1 constitutively expresses NRG1 when untreated (TetOn), but aTC repressed NRG1 expression (TetOff). b, C. albicans in caecum contents stained with AF488 anti-Candida antibody. c, Intestinal fungal burden (mean values ± s.d.). d, Peyer’s patch TFH cells (ICOS+PD-1+CD4+CD3+ live cells) (mean values ± s.d.). e, Peyer’s patch GC B cells (GL-7+Fas+IgD−CD19+ live cells) (mean values ± s.d.). f, Colon LP IgA+ plasma cells (IgA+CD138+CD45+CD3−CD19− live cells) (mean values ± s.d.) quantified from mice monocolonized for four weeks (for c–f, n = 4 mice per group; one experiment). g, Faecal AHR1 qPCR in aTC-treated mice monocolonized with wild-type or TetO-AHR1 (TetOff-AHR1) (wild type n = 3 and TetOff-ALS1 n = 5; one experiment). Mean values ± s.d. h, Fungal burden of wild-type- and TetOff-AHR1-monocolonized mice. i, IgA from wild-type- or TetOff-AHR1-monocolonized mice, j, k, Peyer’s patch TFH cells (j) and Peyer’s patch GC B cells (k) from mice monocolonized with wild type or TetOff-AHR1. l, qRT–PCR from the small intestinal contents of monocolonized mice (for h–l, wild type n = 8 and TetOff-ALS1 n = 10 mice per group from two experiments). m, Intestinal IgA (from C. albicans-monocolonized mice) binding to strains that were cultured untreated or were treated with aTC. n, Human IgA binding to indicated strains cultured without aTC (wild type, ahr1∆/∆, ahr1∆/∆ TetOn-ALS1) or with 5 μg ml−1 aTC (ahr1∆/∆ TetOff-ALS1). IgA binding quantified by flow cytometry (healthy n = 13 and IBD n = 22; one experiment. Samples chosen had enough C. albicans-reactive IgA to bind at least 10% of cultured wild-type C. albicans). P values calculated using one-way ANOVA with Tukey’s test (c–f), two-way ANOVA with Sidak’s test (i), two-sided unpaired t-test (j, k, l), two-sided Mann–Whitney U-test (g) or Friedman test with Dunn’s test (n).
