Extended Data Fig. 7: Acetate increases CCR6-expressing T_FH-like cells in the colon.

a, Representative flow cytometry plots (gated on CD3ε⁺CD4⁺ lymphocytes) depicting CCR6⁺ T cells and CCR6⁺CXCR5⁺ T_FH-like cells in the colon of Ccr6^GFP/+ mice. b, c, The frequency among CD3ε⁺CD4⁺ T cells and the absolute number of CCR6⁺ T cells (b) and CCR6⁺CXCR5⁺ T_FH-like cells (c) in the colon of Ccr6^GFP/+ mice (n = 6 per group). d, Representative flow cytometry plots (gated on CD19⁻CD3ε⁺CD4⁺ lymphocytes) depicting CXCR5⁺PD-1⁺ T_FH-like cells in the colon of wild-type mice. e, f, The frequency among CD3ε⁺CD4⁺ T cells and the absolute number of CXCR5⁺PD-1⁺ T_FH-like cells (e) and RORγt⁺ T_H17 cells (f) in the colon of wild-type mice (n = 5 per group). g, The frequency among CD3ε⁻CD19⁻ lineage marker (lin) negative cells and the absolute number of RORγt⁺ innate lymphoid cells (ILCs) in the colon of wild-type mice (n = 5 per group). Data are mean and s.d. (b, c, e–g). *P < 0.05, **P < 0.01; two-sided Wilcoxon rank-sum test (b, c, e–g). Exact P values are provided in the Source Data.

Source data