Extended Data Fig. 10: Toll-like receptor signalling in CD4 T cells is necessary for acetate to increase colonic IgA production.
From: Acetate differentially regulates IgA reactivity to commensal bacteria
a, Microorganism-reactive SIgA in the caecal contents from Cd3e−/− mice transferred with wild-type (n = 9 per group) or Myd88−/−Ticam1−/− (DKO; n = 8 versus 9) CD4 T cells. b, The absolute number of colonic CD4 T cells in Cd3e−/− mice transferred with wild-type (n = 9 per group) or Myd88−/−Ticam1−/− (n = 8 versus 9) CD4 T cells. c, d, The frequency of caecal SIgA+ bacteria (c) and the absolute number of colonic IgA-producing plasma cells (d) in Cd3e−/− mice monocolonized with E. coli. The mice were transferred with CD4 T cells from either wild-type (n = 4 versus 6) or Myd88−/−Ticam1−/− (n = 4 per group) mice two to three weeks before the dietary intervention. e, f, The frequency of faecal SIgA+ bacteria (e) and the absolute number of colonic IgA-producing plasma cells (f) in Gpr43+/− (n = 5 per group) and Gpr43−/− (n = 5 versus 6) mice. Data are mean and s.d. (a–f). *P < 0.05, **P < 0.01, ***P < 0.001; Kruskal–Wallis test with Dunn’s test (a–f). Pooled data from two independent experiments (a, c, d). Exact P values are provided in the Source Data.
