Extended Data Fig. 5: Astrocyte IL-3 and microglia IL-3Rα production.

a, Il3 expression in astrocytes sorted from WT and 5xFAD mice at 5 and 12 months of age (n = 5 5-month-old WT mice; n = 4 5-month-old 5xFAD mice; n = 3 12-month-old WT mice; n = 4 12-month-old 5xFAD mice). b, Tissue IL-3 levels in various brain regions in WT mice at 4 months of age (n = 4). c, Scheme of daily i.p. LPS injection into WT mice over 4 days and qPCR analysis of C3 and Gfap in sorted astrocytes after LPS injection (n = 4 PBS mice; n = 3 LPS mice for C3; n = 4 LPS mice for Gfap and Il3). Two-tailed Mann–Whitney U-tests. d, CSF IL-3 levels (n = 8 WT PBS mice; n = 6 LPS mice). e, Representative images of GFAP⁺ astrocytes in the cortex of WT, Il3^−/−, 5xFAD, and Il3^−/−5xFAD mice. f, Representative images of Aβ deposits (6E10) and astrocytes (GFAP) in 5xFAD and Il3^−/−5xFAD mice. g, Proportion of IL-3Rα⁺ microglia in the brain of WT mice at various ages (n = 4). h, Il3ra transcript expression in brain homogenate from WT mice at various ages (n = 4). i, Proportion of IL-3Rα⁺ macrophages in heart, liver, lung (interstitial and alveolar), and brain of WT and 5xFAD mice at 8 months of age (n = 3 WT and 5xFAD mice for all tissues except brain where n = 4). One-way ANOVA. *P < 0.05, **P < 0.001. Mean ± s.e.m.