Extended Data Fig. 6: Il3ra expression in microglia and characterization of IL-3Rαhi and IL-3Rαlo microglia.
From: Astrocytic interleukin-3 programs microglia and limits Alzheimer’s disease
a, Analysis of Il3ra and other cytokine receptors expressed in homeostatic, DAM stage 1, and DAM stage 2 microglia (data from ref. 23). b, Gating strategy for IL-3Rαhi and IL-3Rαlo microglia from 5xFAD mice. c, Flow cytometry analysis of IL-3Rαhi and IL-3Rαlo microglia (n = 6 except CD11c and CCL2 where n = 3). d, mRNA transcript expression in sorted IL-3Rαhi and IL-3Rαlo microglia (n = 7 for IL-3Rαlo microglia except that n = 6 for Il6, Itgam, Ptprc, Cd36, and Cd68, and n = 5 for Il10; n = 7 for IL-3Rαhi microglia except that n = 6 for Il6, Ptprc and Cd68, and n = 5 for Il10). *P < 0.05, **P < 0.01, two-tailed Mann–Whitney U-tests. Mean ± s.e.m.
