Extended Data Fig. 7: IL-3 does not influence proliferation, recognition and phagocytosis of Aβ, or production of inflammatory cytokines in microglia.
From: Astrocytic interleukin-3 programs microglia and limits Alzheimer’s disease
a, BrdU incorporation into microglia (n = 6 5xFAD; n = 7 Il3−/−5xFAD). Two-tailed Mann–Whitney U-tests. b, Heat map of microglia RNA-seq expression data for genes important for cell cycle and proliferation. c, Assessment of ex vivo phagocytosis of Aβ42 conjugated to a pH-sensitive dye (PHrodo Red) in sorted microglia lacking Il3 or stimulated with rIL-3 (n = 4). d, Heat map of microglia RNA-seq expression data for genes important for Aβ recognition and phagocytosis. e, Heat map of microglia RNA-seq expression data for inflammatory cytokines. Groups of mice are of evenly mixed sex. Mean ± s.e.m.; ns, not significant.
