Fig. 3: B cell repertoire following vaccination with first dose of BNT162b2.

a, Isotype usage according to unique VDJ sequence in participants <80 (n = 22) or ≥80 years old (n = 28) and association with neutralization of spike pseudotyped virus. Neutralization cut-off for 50% neutralization was set at 20. Mann–Whitney U-test. b, Heat map showing differences in V gene usage between the <80 and ≥80 groups. Mann–Whitney U-test with Benjamini–Hochberg false discovery rate (FDR) correction; *P < 0.1. c, Mean somatic hypermutation for participants <80 or ≥80 years old, grouped according to isotype class. Mann–Whitney U-test. d, Diversity indices for neutralizing and non-neutralizing groups. The inverse is depicted for Simpson’s index. t-test. e, BCR comparison of patients in the two age groups for the first 50 days after vaccination (<80, n = 27; ≥80, n = 5) with public clones known to be associated with SARS-CoV-2 using the CoV-AbDab database¹². Clones from participants and the database were co-clustered based on matching IGHV and IGHJ segments, matching CDR-H3 region length and 85% CDR-H3 sequence amino acid homology. One-sided t-test. For boxplots: centre line, median; box, 25th–75th percentile; whiskers, 1.5× IQR.