Extended Data Fig. 11: Immunofluorescence detection of representative CatSpermasome components in wild-type sperm.

CATSPER1, CATSPER4, CATSPERβ and TMEM249 are mainly distributed in the principal piece of sperm (red fluorescence signal). The sperm head, middle piece and principal piece are indicated by black, blue, and red arrows, respectively. NC, negative control (without primary antibody). Shown here are one of three images taken for each sample. Scale bars, 20 μm.