Extended Data Fig. 5: Analysis of SF3B3-Xon expressed from different promoters.
From: Regulated control of gene therapies by drug-induced splicing
a, Firefly luciferase of the Xon cassettes in response to varying doses of LMI070 relative to Renilla luciferase (grey line). The data are the mean ± s.e.m. of 8 biological replicates. b, Representative RT−PCR for assessment of LMI070-induced pseudo exon expression. Exon inclusion in the Xon cassette was detected using primers flanking the pseudo exon. Data are the mean ± s.e.m. of 8 biological replicates. c, Representative gels from RT−PCR analysis for assessment of the LMI070-induced pseudo exons expressed from the noted promoters in response to varying doses of LMI070. Pseudo exon inclusion was detected using primers binding within the LMI070-induced pseudo exon and the downstream exon. Splicing was quantified and transcript levels presented as the mean ± s.e.m. of 8 biological replicates.
