Extended Data Fig. 7: Fibroblasts.

Cells from clusters 109 to 125 were pulled from the dataset, and a t-SNE embedding was calculated. a, Cells are coloured by their clusters. Putative doublets are grey. b, Cells are coloured by their tissues of origin. c, Cycling cells (>1% cell-cycle UMIs) are coloured blue. d, Expression dot plots are shown for select genes. e, Cells from the indicated embryonic age are coloured blue on each t-SNE. f, Cells are coloured if they express the indicated gene. g, Multiplexed single-molecule RNA fluorescence in situ hybridization for six marker genes (columns) at three different ages (rows). Cartoons on the left indicate the approximate location of each image. Cartoons on the right show an interpretation of the observed gene expression patterns. This experiment was performed once.