Extended Data Fig. 7: Pannexin-1 dependent metabolites enhance Enterobacteriaceae growth.
From: Microbes exploit death-induced nutrient release by gut epithelial cells
a, Left, percentage annexin V staining and TO-PRO-3 dye uptake. n = 4. Right, WT or ΔpflB (CJA071) Salmonella CFU. n = 11 per condition. b, Salmonella CFU after 9 h of anaerobic growth. n = 4 per condition. MeMix-6 formulation used: spermidine (3.0 nM); FBP (5 nM); DHAP (0.36 μM); UDG-glucose (20 nM); GMP (21 nM); IMP (3 3nM). Middle, WT or pflB mutant (CJA071) CFU after 9 h of anaerobic growth Right, Salmonella pflB or cadB gene expression. n = 4 per condition. c, CFU after 9 h of anaerobic growth of wild-type with empty vector (LK010), pflB mutant with empty vector (LK037), or complemented pflB mutant (LK049) with or without 0.2% arabinose. n = 5. d, CFU of the indicated strain of E. coli after 7 h of anaerobic growth. n = 5. e, FBP concentrations. n = 4 per condition. Box plots are as in Fig. 1. ns, P > 0.05. *P ≤ 0.05, **P ≤ 0.005, ***P ≤ 0.0005, two-way ANOVA with Sidak’s multiple comparisons test (a, b (right) c, d), one-way ANOVA with Dunnett’s multiple comparisons test compared with medium control (b (left)) or one-way ANOVA with Tukey’s multiple comparisons test (e)
