Extended Data Fig. 9: Pyroptotic machinery does not impact PflB-dependent fitness.
From: Microbes exploit death-induced nutrient release by gut epithelial cells
a, Schematic of the in vitro model system. b, Total cell death and membrane integrity of HCT116, as measured by flow cytometry, following 24 h of infection. n = 4. c, Aerobic growth of gentamicin-resistant Salmonella (CJA001) measured by OD600. n = 8. d, Expression of pflB and cadB. n = 10. e, Salmonella burden of wild-type (black) or ΔpflB (CJA057, blue) in Casp1/11 mice. n = 7 mice from 2 cohorts. Wild-type and mutant Salmonella connected with dotted lines come from the same mouse. The median competitive index is listed below each tissue. Box plots are as in Fig. 1. Data are mean ± s.e.m. ns, P > 0.05. *P ≤ 0.05, unpaired two-tailed Student’s t-test (c, d), two-tailed Wilcoxon signed rank test with theoretical median of 1 using the calculated competitive index from each mouse (e)
