Extended Data Fig. 11: TNF- and A20-dependent cell death enhances Enterobacteriaceae growth.
From: Microbes exploit death-induced nutrient release by gut epithelial cells
a, Schematic of in vivo infections. b, Activated caspase-3 units. Day 2: n = 4 A20fl/fl control mice, n = 6 Vil-cre+/−A20fl/fl mice from 2 cohorts. Day 3: n = 4 A20fl/fl control mice, n = 4 Vil-cre+/−A20fl/fl mice from 2 cohorts. ns, P > 0.05. *P ≤ 0.05, two-way ANOVA with Sidak’s multiple comparisons test. c, Salmonella burden in the ilea. Day 2: n = 6 A20fl/fl control mice, n = 7 Vil-cre+/−A20fl/fl mice from 2 cohorts. Day 3: n = 9 A20fl/fl control mice, n = 4 Vil-cre+/−A20fl/fl mice from 2 cohorts. d, Competitive index of WT Salmonella vs ΔpflB (CJA057) in the ilea. Day 2: n = 6 A20fl/fl control mice, n = 7 Vil-cre+/−A20fl/fl mice from 2 cohorts. Day 3: n = 7 A20fl/fl control mice, n = 4 Vil-cre+/A20fl/fl mice from 2 cohorts. e, Percentage body weight. n = 12 A20fl/fl control mice. n = 6 Vil-cre+/−A20fl/fl mice from 2 cohorts. f, Caecal weights. n = 12 A20fl/fl control mice. n = 9 Vil-cre+/−A20fl/fl mice from 2 cohorts. g, Salmonella burden. n = 12 A20fl/fl mice. n = 6 Vil-cre+/−A20fl/fl mice from 2 cohorts. Each tissue was analysed separately. Box plots are as in Fig. 1. Data are mean ± s.e.m. ns, P > 0.05. **P ≤ 0.005, ***P ≤ 0.0005, two-tailed Mann–Whitney U-test (c, d, g), two-way ANOVA with Sidak’s multiple comparisons test (e), unpaired two-tailed Student’s t-test (f)
