Extended Data Fig. 3: Mammalian cell death induces time-dependent bacterial outgrowth.
From: Microbes exploit death-induced nutrient release by gut epithelial cells
a, CT26:FADD cells were treated overnight with doxycycline to induce construct expression and were then treated with B/B dimerizer, with or without QVD, and supernatants were collected. Periodic Salmonella growth measurements were quantified by OD600 measurements. n = 5 per condition. b, Salmonella growth. Bacterial growth was assessed via repeated OD600 measurements as indicated. c, Salmonella growth. Bacterial growth was assessed via CFU. Box plots are as in Fig. 1. d, CT26 cell death (left), as determined by flow cytometry, following 24-h treatment with 50 μM PAC-1 (apoptosis inducer) and Salmonella aerobic growth (right) in those supernatants. e, Bacterial aerobic growth (Salmonella (left), E. coli strain HS (middle) and Klebsiella (right)) in CT26 cell supernatants 24 h after 600 mJ cm−2 UV irradiation. f, Bacterial aerobic growth (Salmonella (left), E. coli strain HS (middle) and Klebsiella (right)) in CT26 cell supernatants 24 h after 1 μM staurosporine treatment. g, CT26 cell death (left) and Salmonella aerobic growth (right). CT26 cells were pre-treated for 1 h and maintained with 30 μM QVD as indicated. Data are mean ± s.e.m. *P ≤ 0.05, **P ≤ 0.005, ***P ≤ 0.0005, two-way ANOVA with Tukey’s multiple comparisons test (a, b, d–g)
