Extended Data Fig. 5: Mammalian death-driven bacterial growth is protein-independent.
From: Microbes exploit death-induced nutrient release by gut epithelial cells
a, Schematic of supernatant manipulations after induction of apoptosis that ruled out proteins as responsible for enhanced bacterial growth. b, Total protein levels in media or CT26 supernatants, with or without FBS, at 24 h after staurosporine treatment following indicated proteinase K or filtration strategies as determined by BCA total protein assay. n = 4 per condition. c, CFU of Salmonella. n = 3 per filter size, 9 h of aerobic growth. Median is shown. d, CFU of Salmonella. n = 6 per condition, 9 h of aerobic growth. e, CFU of Salmonella. n = 5 per condition, 9 h of aerobic growth. f, CFU of Salmonella. n = 5 per condition, 9 h of aerobic growth. g, CFU of Salmonella. n = 4 per condition, 9 h of aerobic growth. Box plots as in Fig. 1. Data are mean ± s.e.m. *P ≤ 0.05, **P ≤ 0.005, ***P ≤ 0.0005, multiple two-tailed Student’s t-tests (c), one-way ANOVA with multiple comparisons test (d, e) or unpaired two-tailed Student’s t-test (f, g)
