Extended Data Fig. 11: Comparison of transcriptional responses with other vaccines.

a, Correlation matrix of vaccines on day 1. Spearman’s correlation was computed using mean fold changes over all genes between each pair of vaccines. Circle size and colour represents the correlation coefficient. b, Correlation matrix of vaccines on day 7. c, Circos plot of the overlap across vaccines in enriched BTMs on day 7. GSEA was performed on genes ranked by day 7 versus baseline t-statistic in each vaccine. Each segment of the circle represents one vaccine, and each point in a segment represents a single BTM. Bars in outer circle represent the NES of significantly enriched BTMs (FDR < 0.05). Lines connect BTMs with a significant positive enrichment shared between vaccines. Inner circle boxes and line colours represent the functional groups of the BTMs. d, Heat map of cell cycle, B cell and plasma cell BTMs on day 7. Cell cycle, B cell and plasma cell BTMs that were significantly enriched (FDR < 0.05) on day 7 after either dose of BNT162b2 are shown. Colour represents significant NES. e, Expression of genes in the plasma cell module M156 across different vaccines on day 7 after vaccination. The asterisks denote statistical significance measured by two-sided Wilcoxon test between baseline and day-7 samples within each vaccine group. f, Plasmablast frequencies measured by CyTOF in whole-blood samples. The numbers within plots indicate geometric mean ± s.e.m. at the time points as indicated. Each dot represents an individual in all plots. Blue and red colour denote female and male participants, respectively. Boxes show median and 25th–75th percentiles, and whiskers show the range in all box plots. The statistically significant differences between the peak and baseline time points were measured using two-sided Wilcoxon matched-pairs signed-rank test.