Extended Data Fig. 1: APRIL deficiency does not alter the plaque smooth muscle cell and collagen content, or the numbers of circulating monocytes, B or T lymphocytes in Ldlr^−/− mice.

Ldlr^−/−Tnfsf13^+/+ or Ldlr^−/−Tnfsf13^−/−mice were fed an atherogenic diet for 10 weeks. a, Whole body weight, plasma triglyceride levels and en face lesion size (n = 10 Ldlr^−/−Tnfsf13^+/+ mice, n = 12 Ldlr^−/−Tnfsf13^−/− mice). b–d, Representative photomicrographs of DAPI- (b), α-SMA- (c) and Sirius Red-stained lesions (d) in the aortic origin (left) and dot plots (right) showing the averaged acellular (b; n = 9 Ldlr^−/−Tnfsf13^+/+ mice, n = 12 Ldlr^−/−Tnfsf13^−/− mice, P = 0.036), α-SMA-positive (c, n = 9 Ldlr^−/−Tnfsf13^+/+ mice, n = 10 Ldlr^−/−Tnfsf13^−/− mice) and collagen-positive area normalized to total lesion size (d; n = 10 Ldlr^−/−Tnfsf13^+/+ mice, n = 12 Ldlr^−/−Tnfsf13^−/− mice). e–g, Representative flow cytometry dot plots (top) and quantification (bottom) of the absolute numbers of splenic CD3⁺, CD4⁺ (defined as CD3⁺CD4⁺CD8⁻) and CD8⁺ T cells (defined as CD3⁺CD8⁺CD4⁻) (e), frequencies of peritoneal B-1a (defined as B220^lowCD11b^intCD5⁺), B-1b (defined as B220^lowCD11b^intCD5⁻) and CD23⁺ B-2 (defined as B220^highCD11b⁻CD5⁻CD23⁺) cells (f) and the frequencies of circulating Ly6C^high, Ly6C^int and Ly6C^low monocytes (g) in peripheral blood. All results show mean (two-tailed unpaired Student’s t-test). Scale bars, 200 μm.

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