Extended Data Fig. 5: Anti-APRIL antibodies 108, 2C8 and Apry-1-1 are specific for mouse APRIL.
From: APRIL limits atherosclerosis by binding to heparan sulfate proteoglycans
a, Coomassie blue analyses of anti-mAPRIL mAb 108 and 2C8 under reducing conditions. b, Isotyping of the Fc portions of anti-mAPRIL 108, Apry-1-1 and 2C8. Purified antibodies coated on an ELISA plate were revealed with peroxidase-conjugated antibodies against different isotypes. c, Inhibitory activity of 108 and Apry-1-1 compared to that of TACI-Fc on human and mouse APRIL. Flag–human APRIL and two splice variants of Flag–mouse APRIL (±Ala112) were titrated on BCMA–Fas reporter cells in the presence of a fixed, non-saturating concentration of 108, Apry-1-1 or TACI-Fc. The data show that 108 and Apry-1-1 inhibit both splice variants of mAPRIL at roughly stoichiometric ratios, but do not cross-react with human APRIL. d, ELISA for 2C8 binding to mouse APRIL. Binding of 2C8 to plates coated with human Fc–mouse APRIL was evaluated with a peroxidase-coupled anti-mouse antibody. a, b, Data are representative of two independent experiments.
