Extended Data Fig. 5: Transcript and protein levels of immune-related genes in Col-0 and pad4.
From: The EDS1–PAD4–ADR1 node mediates Arabidopsis pattern-triggered immunity
a, Background levels of a set of immune-related genes in naive Col-0 and pad4. Relative expression was determined by qRT–PCR. Expression was normalized to EF-1α transcript and set relative to Col-0. n = 8 biological replicates from 3 independent experiments. Centre line: median, bounds of box: 25th and 75th percentiles, whiskers: 1.5 * IQR. No significant differences were identified between Col-0 and pad4 (P > 0.05, two-sided Wilcoxon rank sum test with continuity correction). Exact P values are provided in Supplementary Table 5. b, Protein levels of FLS2, BAK1, MPK3, MPK4, and MPK6 are similar in Col-0 and pad4 plants. Leaves were taken from three 6-week-old plants (labelled 1-3) and endogenous protein levels were evaluated by protein blot. Ponceau S-stained RUBISCO large subunit serves as a loading control. For gel source data, see Supplementary Fig. 1. The experiment was repeated at least three times with similar results.
