Extended Data Fig. 4: Target release from hAGO2 and EfPiwi loaded with identical guides.
a, Schematic of pairing between guide RNAs and seed-matched target RNAs used in main text Fig. 2b, c. b, Schematic of pairing between 22 nt guide RNA and target RNAs spanning the seed and central regions. c. Release of 32P-labeled target RNAs from EfPiwi-22 nt guide in the presence of excess unlabeled target RNA over time. d, Release rates of target RNAs from hAGO2-22 nt guide (data from Fig. 2d, left) and EfPiwi-22nt guide (c). Results show hAGO2 and EfPiwi create distinct binding properties for the same guide RNA. All plotted data are the mean values of triplicate measurements. Error bars indicate SD. e, Ribbon representation of hAGO2, EfPiwi, and an overlay illustrating relative positions of the central-gate and seed-gate. In c, d, n = 3 independent experiments, data are mean ± s.d.
