Extended Data Fig. 1: F. novicida induces AIM2-, Pyrin-, ZBP1-mediated caspase-1 activation, cytokine release and cell death.

a, Immunoblot analysis of pro–caspase-1 (CASP1; P45) and cleaved CASP1 (P20) in F. novicida-infected or poly(dA:dT)-transfected wild type (WT) or Aim2^−/− bone marrow-derived macrophages (BMDMs). b, Cell death in BMDMs after F. novicida infection for 16 h. Red indicates dead cells. c, Quantification of the cell death in (b). d–f, Immunoblot analysis of CASP1 (d), cell death images at 16 h post-infection (e), and cell death quantification (f) from WT or Nlrp3^−/− BMDMs after F. novicida infection or LPS plus nigericin (LPS + Ni) treatment. g–i, Immunoblot analysis of CASP1 (g), cell death images at 16 h post-infection (h), and cell death quantification (i) from WT or Nlrc4^−/− BMDMs after F. novicida or Salmonella Typhimurium infection. j–l, Immunoblot analysis of CASP1 (j), cell death images at 16 h post-infection (k), and cell death quantification (l) from WT or Mefv^−/− BMDMs after F. novicida infection or C. difficile Toxin AB⁺ supernatant treatment. m–o, Immunoblot analysis of CASP1 (m), cell death images at 16 h post-infection (n), and cell death quantification (o) from WT or Zbp1^−/− BMDMs after F. novicida or influenza A virus (IAV) infection. a, d, g, j, m, Data are representative of at least three independent experiments. b, e, h, k, n, Images are representative of at least three independent experiments. Scale bar, 50 μm. c, f, i, l, o, Data are mean ± s.e.m. ns, not significant; ****P < 0.0001 (two-tailed t-test; n = 8 from 4 biologically independent samples). Exact P values are presented in Supplementary Table 1. For gel source data, see Supplementary Figure 1.

Source data