Extended Data Fig. 2: Innate immune sensors TLR3, MDA5, NLRP6 and NLRP12 and adaptors Trif and MAVS are not required for caspase-1 activation and cell death after HSV1 and F. novicida infections.

a, Immunoblot analysis of pro–caspase-1 (CASP1; P45) and cleaved CASP1 (P20) in HSV1-infected wild type (WT), Tlr3^−/−, Trif^−/− or Asc^−/− bone marrow-derived macrophages (BMDMs). b, Cell death in BMDMs after HSV1 infection for 16 h. Red indicates dead cells. c, Quantification of the cell death in (b). d–f, Immunoblot analysis of CASP1 (d), cell death images at 16 h post-infection (e), and cell death quantification (f) from WT, Mda5^−/− or Mavs^−/− BMDMs after HSV1 infection. g–i, Immunoblot analysis of CASP1 (g), cell death images at 16 h post-infection (h), and cell death quantification (i) from WT, Nlrp6^−/− or Nlrp12^−/− BMDMs after HSV1 infection. j–l, Immunoblot analysis of CASP1 (j), cell death images at 16 h post-infection (k), and cell death quantification (l) from WT, Tlr3^−/− or Trif^−/− BMDMs after F. novicida infection. m–o, Immunoblot analysis of CASP1 (m), cell death images (n), and cell death quantification (o) from WT, Mda5^−/− or Mavs^−/− BMDMs after F. novicida infection. p–r, Immunoblot analysis of CASP1 (p), cell death images at 16 h post-infection (q), and cell death quantification (r) from WT, Nlrp6^−/− or Nlrp12^−/− BMDMs after F. novicida infection. a, d, g, j, m, p, Data are representative of at least three independent experiments. b, e, h, k, n, q, Images are representative of at least three independent experiments. Scale bar, 50 μm. c, f, i, l, o, r, Data are mean ± s.e.m. ns, not significant (one-way ANOVA with Dunnett’s multiple comparisons test; n = 8 from 4 biologically independent samples). Exact P values are presented in Supplementary Table 1. For gel source data, see Supplementary Figure 1.

Source data