Extended Data Fig. 3: ZBP1 cooperates with Pyrin to drive AIM2-mediated cell death and cytokine release.
From: AIM2 forms a complex with pyrin and ZBP1 to drive PANoptosis and host defence
a, Cell death in bone marrow-derived macrophages (BMDMs) after HSV1 infection with or without colchicine (Col). Red indicates dead cells. Data are representative of at least three independent experiments. Scale bar, 50 μm. b, Quantification of the cell death from (a). Data are mean ± s.e.m. ns, not significant; ****P < 0.0001 (one-way ANOVA with Dunnett’s multiple comparisons test; n = 12 from 3 biologically independent samples). c, Cell death in BMDMs after F. novicida infection with or without Col. Red indicates dead cells. Data are representative of at least three independent experiments. Scale bar, 50 μm. d, Quantification of the cell death from (c). Data are mean ± s.e.m. ns, not significant; ****P < 0.0001 (one-way ANOVA with Dunnett’s multiple comparisons test; n = 9 from 3 biologically independent samples). e–h, Release of IL-1β (e, g) or IL-18 (f, h) following HSV1 (e, f) or F. novicida (g, h) infections with or without Col. Data are mean ± s.e.m. ns, not significant; ****P < 0.0001 (one-way ANOVA with Dunnett’s multiple comparisons test; n = 6 from 3 biologically independent samples). Exact P values are presented in Supplementary Table 1.
