Fig. 2: Pairing between segregated sisters.
From: RecA finds homologous DNA by reduced dimensionality search
a, Cell with −25-kb MalI marker undergoing DSB repair. ParB focus loss and colocalization of MalI foci are annotated. Cell outlines are displayed with a white line. b, Top, cartoon showing the circular E. coli chromosome with the locations of inserted cut site and malO/MalI markers. Bottom, cartoon showing the processing of a DSB in presence of the malO array protected by chi sites. The chi sites prevent RecBCD from degrading the DNA before reaching malO sites. c, Localization of −25-kb MalI foci in cells undergoing DSB. For each cell, the time was aligned by ParB focus loss. n = 654 cells from 3 experiments. d, As c, but for the ygaY MalI marker, on the opposite chromosomal arm to the cut site. n = 753 cells from 2 experiments. e, Distribution of MalI foci colocalization times for −25 kb MalI marker during DSB repair. The solid line shows the gamma fit to the data. n = 508 cells from 3 experiments.
