Extended Data Fig. 7: Additional characterization of DiCas7-11 processing.
From: Programmable RNA targeting with the single-protein CRISPR effector Cas7-11
a, CRISPR array processing assay showing robust processing activity by DiCas7-11 at concentrations ranging from 0 to 233 nM. b, Schematic of in vitro transcription of pre-crRNA and processing by DiCas7-11. c, Sequence of single spacer pre-crRNA, showing locations of mutated bases. d, Processing of transcribed pre-crRNA, showing the effect of single position mutations on processing. e, Processing of transcribed pre-crRNA at different concentrations of DiCas7-11. f, Processing of pre-crRNA by DiCas7-11 in the presence of different ions or chelating agents. CRISPR array processing by DiCas7-11 is ion independent. g, Processing activity of a synthetic DiCas7-11 CRISPR array by DiCas7-11 protein with predicted catalytic processing mutants in the protein insert region with a characteristic KxYxH catalytic triad motif.
