Extended Data Fig. 3: The RNA-guided RNA-targeting Cas7-11 is capable of defence against ssRNA MS2 phage and RNA knockdown in bacteria.
From: Programmable RNA targeting with the single-protein CRISPR effector Cas7-11
a, Schematic of CRISPR array screen of all spacers targeting the MS2 genome. b, Results of the MS2 interference screen shown as box plots. Enrichment of DiCas7-11 spacers in the phage targeting condition denote survival of bacteria and enhanced representation of specific active spacers. Boxes denotes 25th and 75th percentiles with the median marked by the middle line. The whiskers are calculated via the Tukey method (1.5 times the inter-quartile range). Outliers are denoted by blue plus symbols. c, Results of the MS2 interference screen showing enrichment of DiCas7-11a non-targeting spacers across varying phage dilution amounts as box plots. Boxes denotes 25th and 75th percentiles with the median marked by the middle line. The whiskers are calculated via the Tukey method (1.5 times the inter-quartile range). Outliers are denoted by blue plus symbols. d, Number of DiCas7-11a spacers that display survival enrichment over a threshold of 1.7 across different phage dilution conditions. e, Quantification of resistance conferred by top MS2-targeting DiCas7-11 spacers compared against a panel of 4 non-targeting spacers. Resistance is quantified as the highest surviving titer of MS2 phage that generates plaques in the dilution assay. f, Quantification of resistance conferred by two top MS2-targeting DiCas7-11 spacers compared against a panel of 6 non-targeting spacers. Resistance is quantified as the highest surviving titer of MS2 phage that generates plaques in the dilution assay. Data are mean ± s.e.m.; n = 3.
