Extended Data Fig. 7: Duloxetine induces a shift in metabolite secretion.
From: Bioaccumulation of therapeutic drugs by human gut bacteria
a, Effect of duloxetine treatment on the exo-metabolome of six gut bacterial strains. Shown is the distribution of individual samples over the first two principle components. Principle Component Analysis (PCA) was performed on untargeted FIA–MS data (Methods). The numbers in parentheses of PC1 and PC2 mark the corresponding explained variance for the first and the second principle component, respectively. The dotted block arrow marks the duloxetine induced shift in exo-metabolome of C. saccharolyticum. b, Duloxetine concentration dependent changes in the C. saccharolyticum exo-metabolome. The ion mapping to the deprotonated duloxetine was removed from the PCA analysis shown in a and b. c, The signal for the closest matching ion for deprotonated duloxetine [M-H]- from the exometabolomics data (m/z 296.110079) plotted against initial duloxetine concentration. Data from all six species are pooled together (n = 24 for each initial duloxetine concentration). Overlaid box plots show the interquartile range (IQR), the median value and whiskers extending to include all the values less than 1.5 × IQR away from the 1st or 3rd quartile, respectively. d, Duloxetine signal in the FIA–MS data stratified by species. The signal for the closest matching ion for deprotonated duloxetine [M-H]- from the exometabolomics data (FIA–MS) (m/z 296.110079) plotted against initial duloxetine concentration. Thick transparent line traces medians of replicates (n = 4) at each initial concentration. The dotted lines show linear regression fit.
