Extended Data Fig. 4: NMR measurements showing unmodified duloxetine recovered from bacterial pellet.

Bacterial cells were incubated with the drug for 4 h in PBS buffer prior to recovery. a, Illustration of the experimental procedure marking the sample collection points. b, NMR spectra of recovered duloxetine from different fractions of E. coli IAI1 and C. saccharolyticum preincubated in PBS. The reference spectrum was scaled to the amount present in the sample to assess the relative amount of free duloxetine present in respective samples. Resonances appearing to be out of phase and strong baseline distortions are due to the presence of large solvent signals outside the displayed chemical shift range.