Extended Data Fig. 5: Duloxetine bioaccumulation by E. coli IAI1 in GMM and recovery from pellet.
From: Bioaccumulation of therapeutic drugs by human gut bacteria
a, Procedure and collected samples: S0-S4. b, Recovered duloxetine from different samples (S0-S4) collected as described in a. Different starting duloxetine concentrations, between 0-70 µM, were used. S0 = medium without bacteria (drug only control), S1 = total culture (medium plus bacteria), S2 = supernatant, S3 = wash (pellet was washed with PBS, no drug was found therein supporting intracellular accumulation), S4 = washed pellet. n = 3, error bars = SD, central squares mark the mean. c, MS/MS spectra of duloxetine standard (bottom) and duloxetine detected in a S1 sample (top).
