Extended Data Fig. 5: Pathway analysis of the two MIMS populations and comparison with DAM.
From: A lymphocyte–microglia–astrocyte axis in chronic active multiple sclerosis
a, b, Gene enrichment and pathway analysis of the top 100 differentially expressed genes are shown for MIMS-foamy and MIMS-iron populations, separately. Using the gProfileR package, different sources were compared (GO:BP, GO:CC, GO:MF, KEGG). Only significant pathway terms are plotted in the graphs (p < 0.05, correction methods "g_SCS"). See the text for interpretation. c, Homeostatic microglia vs. MIMS vs. DAM: direct comparison of two single-cell RNA-seq datasets (MS [current work] and a mouse AD model [5XFAD]). Data for the AD model are derived from supplementary table 2 of ref. 18. In each dataset, only significant differentially expressed genes in the comparison between homeostatic vs. MIMS and homeostatic vs. DAM were retained (p < 0.001). Volcano plots report overlapping genes for each microglia phenotype. MS, multiple sclerosis; MIMS, microglia inflamed in MS; AD, Alzheimer disease; DAM, degenerative disease-associated microglia.
