Extended Data Fig. 5: Tau pathology in limbic-predominant neuronal inclusion body 4R tauopathy (LNT, PSP-F case 2).

a, Low power view of hippocampus stained with antibody AT8 (pS202/pT205tau). b, Low power view of frontal cortex stained with AT8. c, Higher power view of hippocampus stained with AT8. d, AT8-positive globular astrocyte in hippocampus. e, AT8-positive neurons in hippocampus. f, Hippocampus stained with antibody RD4 (specific for 4R tau). g, Gallyas-Braak silver-positive neurons and glial cells in hippocampus. h, Hippocampus stained with antibody RD3 (specific for 3R tau). i, Higher-power view of frontal cortex stained with AT8. j, AT8-positive globular astrocyte in frontal cortex. k, AT8-positive neurons in frontal cortex. l, Frontal cortex stained with RD4. m, Gallyas-Braak silver-positive neurons and glial cells in frontal cortex. n, Frontal cortex stained with RD3. Representative images are shown. Similar results were obtained using a minimum of six additional stained sections for each panel. Scale bars: 400 μm in (a); 200 μm in (b), 50 μm in (c, f, g, h, i, l, m, n); 10 μm in (d, e, j, k).