Extended Data Fig. 1: Cryo-EM images and single-particle reconstruction of the CG–LHCGR–Gs complex.
From: Structures of full-length glycoprotein hormone receptor signalling complexes
a, Size-exclusion chromatography elution profile and SDS-PAGE of the CG–LHCGR–Gs complex. Red star indicates the monomer peak of the complex. For gel source data, see Supplementary Fig. 1 (Experiments were repeated three times with similar results). b, Cryo-EM micrograph, reference-free 2D class averages, and flowchart of cryo-EM data analysis of the CG–LHCGR–Gs complex. c, Cryo-EM map of the CG–LHCGR–Gs complex coloured by local resolutions from 2.0 Å (blue) to 5.0 Å (red). The ‘gold-standard’ Fourier shell correlation (FSC) curve indicates that the overall resolution of the electron density map of the CG–LHCGR–Gs complex is 3.9 Å.
