Extended Data Fig. 2: Cryo-EM image and single-particle reconstruction of the Org43553–CG–LHCGR–Gs complex.
From: Structures of full-length glycoprotein hormone receptor signalling complexes
a, Size-exclusion chromatography elution profile and SDS–PAGE of the Org43553–CG–LHCGR–Gs complex. Red star indicates the monomer peak of the two complex. For gel source data, see Supplementary Fig. 1 (Experiments were repeated three times with similar results). b, c, Cryo-EM micrograph, reference-free 2D class averages, and flowchart of cryo-EM data analysis of the Org43553–CG–LHCGR–Gs complex. Org43553–CG–LHCGR–Gs complex coloured by local resolutions from 2.0 Å (blue) to 5.0 Å (red).The ‘gold-standard’ Fourier shell correlation (FSC) curves indicate that the overall resolution of the Org43553–CG–LHCGR–Gs complex is 3.18 Å (Focused refinement) and 3.3 Å (Global refinement), respectively. d, Cryo-EM density map and model of the Org43553–CG–LHCGR–Gs complex. The regions of the cryo-EM density map with all transmembrane helices, H8 and hinge C terminus are shown.
