Fig. 3: ACE2 overexpression influences neutralization by different classes of monoclonal antibodies.
From: Lectins enhance SARS-CoV-2 infection and influence neutralizing antibodies
a, Surface rendering of a composite model of SARS-CoV-2 S bound to S309 (purple), S2E12 (magenta) and S2X333 (orange)7,25,26. The three SARS-CoV-2 S protomers are coloured light blue, gold and pink with N-linked glycans rendered dark blue. b, SARS-CoV-2 S or RBD binding to the indicated cell lines was quantified by flow cytometry. A, ACE2; T, TMPRSS2. Graph shows mean of two biological replicates. MFI, mean fluorescence intensity. c, d, A panel of seven cell lines was infected with SARS-CoV-2-Nluc (c) or VSV-SARS-CoV-2 pseudovirus (d) in the presence of S309, S2E12 or S2X333. Luciferase signals were quantified 24 h after infection (n = 3 biologically independent replicates).
