Extended Data Fig. 3: Characterization of SARS-CoV-2-susceptible cell lines.
From: Lectins enhance SARS-CoV-2 infection and influence neutralizing antibodies
a, SARS-CoV-2 neutralization with S309, S2E12 and S2X333 on Vero E6 or Vero E6-TMPRSS2 cells. Cells were infected with SARS-CoV-2 (isolate USA-WA1/2020) at MOI 0.01 in the presence of the respective mAbs. Cells were fixed 24h post infection, viral nucleocapsid protein was immunostained and quantified (n = 3 biologically independent replicates). b, IFA images of experiment in (a): SARS-CoV-2 neutralization with 10 µg/ml of S309, S2E12 and S2X333 on Vero E6 or Vero E6-TMPRSS2 cells (scale bar: 100 µm). c, Purified, fluorescently-labelled SARS-CoV-2 spike protein or RBD protein was incubated with the indicated cell lines and protein binding was quantified by flow cytometry. d, Cellular ACE2 and TMPRSS2 transcripts were quantified by RT-qPCR. Expression levels were adjusted by HPRT levels and are presented as normalized to HeLa cell levels, showing the mean of technical triplicates. e, Correlation analysis between ACE2 transcript levels and maximum antibody neutralization in all SARS-CoV-2-susceptible cell lines. Nonparametric, two-tailed Spearman correlation was calculated using GraphPad Prism.
